Plant regeneration from rice (Oryza sativaL.) embryogenic suspension cells cryopreserved by vitrification
✍ Scribed by Chun-Nong Huang; Jun-Hui Wang; Qiu-Sheng Yan; Xue-Qin Zhang; Qin-Feng Yan
- Publisher
- Springer
- Year
- 1995
- Tongue
- English
- Weight
- 767 KB
- Volume
- 14
- Category
- Article
- ISSN
- 0721-7714
No coin nor oath required. For personal study only.
✦ Synopsis
Rice cells were precultured for 10 d in medium containing 60 g/L sucrose and subsequently for 1 d in medium supplemented with 0. 4 M sorbitol. After loading with 25%PVS2 at 22°C for 10 min and dehydration in 100%PVS2 at 0°C for 7. 5 min,they were plunged into liquid nitrogen directly. Survival was 45. 0 ±5.1% (based on the reduction of triphenyl tetrazolium chloride)following warming and unloading. For regrowth, cells were plated on semi-solid medium replenished with 40%(w/v) starch for 2d prior to reculture. Cell suspensions were reestablished and plants were regenerated from recovered cells. Twenty eight plants set seeds in the greenhouse.