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Simple dehydration treatment promotes plantlet regeneration of rice (Oryza sativaL.) callus

โœ Scribed by Masayoshi Tsukahara; Takayasu Hirosawa

Publisher
Springer
Year
1992
Tongue
English
Weight
488 KB
Volume
11
Category
Article
ISSN
0721-7714

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โœฆ Synopsis

To increase plantlet regeneration frequency, rice callus was dehydrated in a Petri dish with a single layer of filter paper prior to transfer to the regeneration medium. With a 24 h dehydration treatment, the regeneration frequency was increased to 47 %, while the regeneration frequency of the untreated control was less than 5 %. This relatively simple method provides an alternative method for improving the regeneration frequency of rice callus.

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Plant cell and tissue culture are likely to continue to be of key importance in the application of molecular biology to crop improvement. Strategies to regenerate plants at high efficiency from tissue culture of cereals have been evolving steadily during the past decades (for a review see Morrish et