Improved estimation of DNA fragment lengths from agarose gels
β Scribed by Henry E. Schaffer; Ronald R. Sederoff
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 728 KB
- Volume
- 115
- Category
- Article
- ISSN
- 0003-2697
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## Abstract A simple elution tube has been constructed which allows reliable electroelution of DNA restriction fragments from agarose and acrylamide gels. This method enables the recovery of DNA fragments of 50 to over 50 000 base pairs from cylindrical or slab gels with yields between 70 % to 90 %
After electrophoresis, DNA can be efficiently recovered by solubilization of agarose gels with NaC104, followed by retention of DNA on glass fiber filters. After removal of the NaCIO, by ethanol, the DNA can be extracted with a low salt buffer.