Identification of gcpE as a novel gene of the 2-C-methyl-D-erythritol 4-phosphate pathway for isoprenoid biosynthesis in Escherichia coli
✍ Scribed by Narciso Campos; Manuel Rodrı́guez-Concepción; Myriam Seemann; Michel Rohmer; Albert Boronat
- Book ID
- 117102643
- Publisher
- Elsevier Science
- Year
- 2001
- Tongue
- English
- Weight
- 183 KB
- Volume
- 488
- Category
- Article
- ISSN
- 0014-5793
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📜 SIMILAR VOLUMES
In the bacterium Escherichia coli, gcpE is an essential gene in the methylerythritol phosphate pathway for isoprenoid biosynthesis. Incubation of [1-3 H]methylerythritol with an E. coli mutant defective in the gcpE gene resulted in the accumulation of [1-3 H]methylerythritol 2,4-cyclodiphosphate. Th
Optically pure 2-C-methyl-d-erythritol 4-phosphate, a key intermediate in the mevalonate-independent route for isoprenoid biosynthesis, is conveniently synthesized from 1,2-O-isopropylidene-a-d-xylofuranose, including the possibility of radiolabeling of this substrate.