Identification of concanavalin A-binding proteins after sodium dodecyl sulfate-gel electrophoresis and protein blotting
✍ Scribed by Richard Hawkes
- Publisher
- Elsevier Science
- Year
- 1982
- Tongue
- English
- Weight
- 769 KB
- Volume
- 123
- Category
- Article
- ISSN
- 0003-2697
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Capillary sodium dodecyl sulfate-gel electrophoresis, a one-dimensional version of the well-established planar analytical method of SDS-polyacrylamide gel electrophoresis, has proven a powerful new microanalytical method for the separation of protein molecules according to their size. In this paper
2), the methodology of this application has changed High-molecular-weight proteins often blot onto nilittle since it was originally introduced for use with trocellulose membranes poorly following sodium dourea-or SDS 2 -containing polyacrylamide gels (1). Nudecyl sulfate-polyacrylamide gel electroph