Comparison of the Separation of Proteins by Sodium Dodecyl Sulfate-Slab Gel Electrophoresis and Capillary Sodium Dodecyl Sulfate-Gel Electrophoresis

✍ Scribed by A. Guttman; J. Nolan

• Publisher: Elsevier Science
• Year: 1994
• Tongue: English
• Weight: 391 KB
• Volume: 221
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1006/abio.1994.1413

✦ Synopsis

Capillary sodium dodecyl sulfate-gel electrophoresis, a one-dimensional version of the well-established planar analytical method of SDS-polyacrylamide gel electrophoresis, has proven a powerful new microanalytical method for the separation of protein molecules according to their size. In this paper the planar SDSPAGE and the capillary SDS-gel electrophoresis of different proteins are compared with respect to performance, precision, and efficiency. Molecular weight versus migration distance and migration time curves for 65 proteins, running from 14,200 to (205,000 \mathrm{Da}), are given for both methods. 1994 Academic Press, Inc.