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Hydrosoluble fluorogenic substrates for plasmin

✍ Scribed by Marzia Harnois-Pontoni; Michel Monsigny; Roger Mayer

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 810 KB
Volume: 193
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90017-n

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✦ Synopsis

New hydrosoluble fluorogenic substrates for plasmin gluconoylpeptidyl-3-amido-9-ethylcarbazole were synthesized. The substitution of the N-terminal end of the peptides by a gluconoyl group prevents the substrates from aminopeptidase degradation and highly increases their hydrosolubility. The substitution of the peptide C-terminal end by a 3-amino-9-ethylcarbazole group leads to substrates suitable for direct fluorometric assay of plasmin present in cell supernatants or in cell lysates. On the basis of the kinetic parameters of the substrate hydrolysis by plasmin, it was found that D amino acids in the P2 position decrease systematically the kinetic constants of the substrates. The L configuration of the P2 amino acid appears therefore as essential in optimum substrates for plasmin.

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