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Internally Quenched Fluorogenic Substrate for Furin

✍ Scribed by H. Angliker; U. Neumann; S.S. Molloy; G. Thomas

Publisher: Elsevier Science
Year: 1995
Tongue: English
Weight: 300 KB
Volume: 224
Category: Article
ISSN: 0003-2697
DOI: 10.1006/abio.1995.1058

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✦ Synopsis

A new substrate for furin, Abz-Arg-Val-Lys-ArgGly-Leu-Ala-Tyr (\left(\mathrm{NO}{2}\right))-Asp-OH, has been synthesized and characterized. The peptide is an internally quenched fluorogenic substrate. The kinetic parameters are (K{m}=3.8 \mu \mathrm{M}, k_{\text {cat }}=29.3 \mathrm{~s}^{-1}), and (k_{\text {cat }} / K_{M}=) (7,710,000 \mathrm{~m}^{-1} \mathrm{~s}^{-1}). The substrate is efficiently cleaved by furin; its (k_{\mathrm{cat}} / \boldsymbol{K}_{M}) value is over 2000 -fold higher than that of the commonly used substrate Boc-Arg-ValArg-Arg-AMC. ic 1995 Academic Press, Inc.

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