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A fluorogenic substrate for angiotensin-converting enzyme

✍ Scribed by Anders Persson; Irwin B. Wilson

Publisher
Elsevier Science
Year
1977
Tongue
English
Weight
406 KB
Volume
83
Category
Article
ISSN
0003-2697

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✦ Synopsis

A fluorogenic substrate for the angiotensin-converting enzyme (EC 3.4.15.1) is presented: para-nitrobenzyloxycarbonylglycyl-~-tryptophylglycine.

The assay is based upon relief of the internal quenching of tryptophan fluorescence by the nitrobenzyl group when the substrate is enzymatically hydrolyzed to produce para-nitrobenzyloxycarbonylglycine and tryptophylglycine. The new substrate has a K, of 9 x 10e4 M at 37Β°C and pH 7.4 and is rapidly hydrolyzed in comparison with other substrates. We also describe the use of fluorescamine to assay the dipeptides released from N-blocked substrates by the converting enzyme.

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