Synthesis and Hydrolysis by Cysteine and Serine Proteases of Short Internally Quenched Fluorogenic Peptides

Nonspecific protease-catalyzed hydrolysi

Nonspecific protease-catalyzed hydrolysis/synthesis of a mixture of peptides: Product diversity and ligand amplification by a molecular trap

✍ Patrick G. Swann; Rose A. Casanova; Archana Desai; Mary M. Frauenhoff; Minka Urb 📂 Article 📅 1996 🏛 Wiley (John Wiley & Sons) 🌐 English ⚖ 804 KB

We sought to develop a peptide library in solution and dynamically screen this library for peptides that would bind to macromolecules of interest. Peptide diversity was achieved in an initial stock solution of peptides by using proteases under conditions in which both hydrolysis and synthesis occurr