✦ LIBER ✦

Fluorogenic peptide amide substrates for the estimation of plasminogen activators and plasmin

✍ Scribed by W. Nieuwenhuizen; G. Wijngaards; E. Groeneveld

Publisher: Elsevier Science
Year: 1977
Tongue: English
Weight: 312 KB
Volume: 83
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(77)90519-x

No coin nor oath required. For personal study only.

✦ Synopsis

Fluorogenic substrates, i.e., t-BOC-L-valyl-glycyl-L-arginine P-naphthylamide (II) and L-valyl-glycyl-L-arginine P-naphthylamide (III) were synthesized. The kinetic constants of the hydrolysis of these substrates and of commercial glycyl-L-arginine P-naphthylamide (I) by plasmin, urokinase, and plasminogen activator from uterine tissue have been determined. It appears that all three peptide substrates are very suitable for assaying extremely low amounts of plasmin and urokinase. The tissue activator is active only against substrate II bearing an amino-terminal blocking group. This difference allows discrimination between tissue activator activity and other proteolytic activities; e.g. plasmin, urokinase, and trypsin.

📜 SIMILAR VOLUMES

A Spectrophotometric Assay for the Deter

A Spectrophotometric Assay for the Determination of the Catalytic Efficiency of Plasminogen Activators Using a Slowly Hydrolyzed Plasmin Substrate

✍ M.J.M. Castro; I.B. Kingston; S. Anderson 📂 Article 📅 1995 🏛 Elsevier Science 🌐 English ⚖ 591 KB

A simple spectrophotometric assay for the determination of the catalytic efficiency and activity of plasminogen activators is presented. The assay system contains activator, plasminogen, and the chromogenic substrate \(N\)-benzoyl-L-arginine-p-nitroanilide (BAPA). Plasmin production is monitored con

Sympathectomy decreases and adrenergic s

Sympathectomy decreases and adrenergic stimulation increases the release of tissue plasminogen activator (t-PA) from blood vessels: Functional evidence for a neurologic regulation of plasmin production within vessel walls and other tissue matrices

✍ Tao Peng; Xi Jiang; Yafei Wang; Arthur Hand; Concettina Gillies; Robert E. Cone; 📂 Article 📅 1999 🏛 John Wiley and Sons 🌐 English ⚖ 287 KB 👁 2 views

Our recent morphologic studies indicated that peripheral nervous system (PNS) adrenergic neurons synthesize, transport, and store the serene protease, tissue plasminogen activator (t-PA) in axon terminals, many of which innervate vessel walls. Sympathoadrenal stimulation induces a surge of t-PA from

A Nonradioactive Fluorescent Gel-Shift A

A Nonradioactive Fluorescent Gel-Shift Assay for the Analysis of Protein Phosphatase and Kinase Activities toward Protein-Specific Peptide Substrates

✍ M.P. Lutz; D.I. Pinon; L.J. Miller 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 613 KB