A Nonradioactive Fluorescent Gel-Shift Assay for the Analysis of Protein Phosphatase and Kinase Activities toward Protein-Specific Peptide Substrates

Nonradioactive Assay for Protein Phospha

Nonradioactive Assay for Protein Phosphatase 2B (Calcineurin) Activity Using a Partial Sequence of the Subunit of cAMP-Dependent Protein Kinase as Substrate

✍ A. Enz; G. Shapiro; A. Chappuis; A. Dattler 📂 Article 📅 1994 🏛 Elsevier Science 🌐 English ⚖ 500 KB

Currently, protein phosphatase 2B (calcineurin) activity is assayed based on release of [32P]phosphate from a 19-amino acid peptide (partial sequence of the regulatory subunit of cAMP-dependent protein kinase) following its [32P]ATP phosphorylation using the catalytic subunit of cAMP-dependent prote