High-level expression ofClostridium thermocellumcellulase genes inEscherichia coli
β Scribed by Wolfgang H. Schwarz; Silke Schimming; Walter L. Staudenbauer
- Publisher
- Springer
- Year
- 1987
- Tongue
- English
- Weight
- 619 KB
- Volume
- 27
- Category
- Article
- ISSN
- 1432-0614
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The genes for ferredoxin from heterocysts (fdx H) and vegetative cells (pet F) ofAnabaena sp. strain 7120 were subcloned into plasmid pUC 18/19. Both genes were expressed in Escherichia coli at high levels ( ~ 10 ~o of total protein). Pet F could be expressed from its own promoter. The ferredoxins w
The high-level expression plasmid for streptokinase, pSK100, has been constructed. It contains a tac promoter, an ompA signal sequence, a streptokinase structural gene(skc) and a rrnBT1T2 transcription terminator. E. coli JM109 carrying pSK100 produced about 5,000IU of streptokinase per 1 ml of LB-a
## A thermophilic Bacillus sp. producing xylose (glucose) isomerase has been isolated. Its xy/A gene when cloned in Escherichia co/i and expressed gave 37.5 and 12.8 units/ rng, protein respectively for xylose and glucose isomerase activities at 85 C. A single heat treatment of the crude extract p