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Expression ofAnabaenaferredoxin genes inEscherichia coli

✍ Scribed by H. Böhme; R. Haselkorn

Publisher
Springer
Year
1989
Tongue
English
Weight
480 KB
Volume
12
Category
Article
ISSN
0167-4412

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✦ Synopsis

The genes for ferredoxin from heterocysts (fdx H) and vegetative cells (pet F) ofAnabaena sp. strain 7120 were subcloned into plasmid pUC 18/19. Both genes were expressed in Escherichia coli at high levels ( ~ 10 ~o of total protein). Pet F could be expressed from its own promoter. The ferredoxins were correctly assembled to the holoprotein. Heterocyst ferredoxin was purified from E. coli extracts on a large scale.

Its biochemical and biophysical properties were identical to those of the authentic ferredoxin, isolated from Anabaena heterocysts. * This paper is dedicated to Prof. A. Trebst on the occasion of his 60th birthday.

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