Cloning and expression ofClostridium stercorariumcellulase genes inEscherichia coli
β Scribed by Wolfgang H. Schwarz; Sigrid Jauris; Monika Kouba; Karin Bronnenmeier; Walter L. Staudenbauer
- Publisher
- Springer Netherlands
- Year
- 1989
- Tongue
- English
- Weight
- 345 KB
- Volume
- 11
- Category
- Article
- ISSN
- 0141-5492
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
The coding sequence for Spirulina platensis acetohydroxy acid synthase (AHAS, EC 4.1.3.18) is shown to be contained within a 4.2 Kb ClaI fragment (ilvX) that has been cloned from a recombinant lambda library. This fragment was able to complement a suitable mutant of Escherichia coli when inserted in
The genes for ferredoxin from heterocysts (fdx H) and vegetative cells (pet F) ofAnabaena sp. strain 7120 were subcloned into plasmid pUC 18/19. Both genes were expressed in Escherichia coli at high levels ( ~ 10 ~o of total protein). Pet F could be expressed from its own promoter. The ferredoxins w