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High level expression of a thermostableBacillusxylose (glucose) isomerase inEscherichia coli

✍ Scribed by Liao Wuxiang; Kandiah Jeyaseelan

Publisher
Springer Netherlands
Year
1993
Tongue
English
Weight
348 KB
Volume
15
Category
Article
ISSN
0141-5492

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✦ Synopsis

A thermophilic

Bacillus sp. producing xylose (glucose) isomerase has been isolated. Its xy/A gene when cloned in Escherichia co/i and expressed gave 37.5 and 12.8 units/ rng, protein respectively for xylose and glucose isomerase activities at 85 C. A single heat treatment of the crude extract purified the enzyme further yielding the highest ever recorded activities of 150 and 49.02 units /mg protein.

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