𝔖 Bobbio Scriptorium
✦   LIBER   ✦

High-level C5a gene expression and recovery of recombinant human C5a fromEscherichia coli

✍ Scribed by K. W. Mollison; T. A. Fey; R. A. Krause; W. Mandecki; J. L. Fox; G. W. Carter

Publisher
SP Birkhäuser Verlag Basel
Year
1987
Tongue
English
Weight
324 KB
Volume
21
Category
Article
ISSN
1420-908X

No coin nor oath required. For personal study only.

📜 SIMILAR VOLUMES

High level expression and crystallizatio
High level expression and crystallization of recombinant human cathepsin S
✍ Dieter Brömme; Mary E. Mcgrath 📂 Article 📅 2008 🏛 Cold Spring Harbor Laboratory Press 🌐 English ⚖ 274 KB

## Abstract We have expressed active human cathepsin S to 60 mg/L in Sf9 cells using a baculovirus system. Production of milligram quantities has facilitated crystallographic studies to determine the structure of this enzyme, which has unique properties among lysosomal cysteine proteinases. Recombi

High level expression and simple purific
High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli
✍ Soo-Hyung Kang; Kyu-Heum Na; Jang-Hyeon Park; Choong-II Park; Se-Yong Lee; Young 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 345 KB

A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of