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High level expression and simple purification of recombinant human granulocyte colony-stimulating factor in E. coli

✍ Scribed by Soo-Hyung Kang; Kyu-Heum Na; Jang-Hyeon Park; Choong-II Park; Se-Yong Lee; Young-Ik Lee

Publisher
Springer Netherlands
Year
1995
Tongue
English
Weight
345 KB
Volume
17
Category
Article
ISSN
0141-5492

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✦ Synopsis

A human granulocyte colony-stimulating factor (hG-CSF) gene was syntheeized and inserted into a trp expression vector for overexpression in E. cm?. A strong expression vector was constructed, and a simple purification procedure including in vitro refolding was estabiliehed. The final productivity of hG-CSF was 500-600~ per ! culture, and the purified hG-CSF showed the proliferation of neutrophile in uivo assays

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## Abstract ## BACKGROUND. Ewing sarcoma (ES) is a highly vascular malignancy. It has been demonstrated that both angiogenesis and vasculogenesis contribute to the growth of ES tumors. Granulocyte‐colony‐stimulating factor (G‐CSF), a cytokine known to stimulate bone marrow (BM) stem cell productio