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High-level expression and secretion of streptokinase inEscherichia coli

✍ Scribed by Jae Hyeong Ko; Do Keun Park; Il Chul Kim; Si Hyoung Lee; Si Myung Byun

Publisher: Springer Netherlands
Year: 1995
Tongue: English
Weight: 364 KB
Volume: 17
Category: Article
ISSN: 0141-5492
DOI: 10.1007/bf00143093

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✦ Synopsis

The high-level expression plasmid for streptokinase, pSK100, has been constructed. It contains a tac promoter, an ompA signal sequence, a streptokinase structural gene(skc) and a rrnBT1T2 transcription terminator. E. coli JM109 carrying pSK100 produced about 5,000IU of streptokinase per 1 ml of LB-ampicillin media. About 95% of the expressed streptokinase was secreted into the periplasmic and extracellular fractions. The recombinant streptokinase in high yield and purity may be a potential alternative source for the therapeutic agent.

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