Maximizing the expression of recombinant Kringle 1 (Streptokinase) synthesized inEscherichia coli. Influence of culture and induction conditions
✍ Scribed by R. E. Narciandi; F. J. Mörbe; D. Riesenberg
- Publisher
- Springer Netherlands
- Year
- 1996
- Tongue
- English
- Weight
- 339 KB
- Volume
- 18
- Category
- Article
- ISSN
- 0141-5492
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✦ Synopsis
We examined the influence of culture conditions on the production of the recombinant fused protein Kringle 1 (Streptokinase) (KI(SK)) expressed in E. co/i under the control of the Ipp-lac promoter. The growth and the protein production were severely inhibited at glucose concentrations higher than 5 g/L. The highest yield of KI(SK) from the soluble extract was obtained using synthetic medium. Concentrations of inducer between 0.1 and 1 mM of IPTG did not significantly affect the production level of KI(SK) or the final cell density. Under optimal conditions the KI(SK) protein was expressed in an intracellular soluble form at about 3-4% of the total cellular protein.