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High expression of cytochrome P450 2a-5 (coumarin 7-hydroxylase) in mouse hepatomas

✍ Scribed by Valery Kobliakov; Ludmila Kulikova; Dimitri Samoilov; Matti A. Lang

Book ID
102502403
Publisher
John Wiley and Sons
Year
1993
Tongue
English
Weight
421 KB
Volume
7
Category
Article
ISSN
0899-1987

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✦ Synopsis

Abstract

A high level of Cyp2a‐5 was found in spontaneous and transplanted mouse hepatomas compared with normal liver. Increased expression of Cyp2a‐5 was associated with an increase in coumarin 7‐hydroxylation, a marker activity of Cyp2a‐5, and the corresponding mRNA, suggesting that regulation of Cyp2a‐5 in hepatomas is pretranslational. In contrast, the total P450 content and arylhydrocarbon hydroxylase and amidopyrene demethylase activities decreased. Pyrazole, a strong inducer of Cyp2a‐5 in normal mouse livers, also increases this isozyme in hepatomas. A parallel increase in the corresponding mRNA suggests that pyrazole, like the formation of hepatomas, affects the regulation of Cyp2a‐5 pretranslationally.

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