Growth regulation of WI38 cells in a serum-free medium
โ Scribed by Paul D. Phillips; Vincent J. Cristofalo
- Book ID
- 115891444
- Publisher
- Elsevier Science
- Year
- 1981
- Tongue
- English
- Weight
- 471 KB
- Volume
- 134
- Category
- Article
- ISSN
- 0014-4827
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
A rat ovarian tumorous cell line (OVlN) has been isolated and established in tissue culture whose growth can be controlled by the addition of epidermal growth factor (EGF) and transferrin to serum-free medium. EGF at 1 ng/ml (1.5 x 10-l0M) stimulates growth about 300% in a six-day incubation assay.
Madin Darby canine kidney cells can grow in synthetic medium supplemented with 5 factors ~ insulin. transferrin, prostaglandin E , , hydrocortisone and triiodothyronine ~-as a seruni substitute. These 5 factors permit growth for one month in the absence of serum, and a growth rate equivalent t o tha