✦ LIBER ✦

Growth of kidney epithelial cells in hormone-supplemented, serum-free medium

✍ Scribed by Taub, Mary ;Sato, Gordon H.

Publisher: Wiley (John Wiley & Sons)
Year: 1979
Tongue: English
Weight: 747 KB
Volume: 11
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400110210

No coin nor oath required. For personal study only.

✦ Synopsis

Madin Darby canine kidney cells can grow in synthetic medium supplemented with 5 factors ~ insulin. transferrin, prostaglandin E , , hydrocortisone and triiodothyronine ~-as a seruni substitute. These 5 factors permit growth for one month in the absence of serum, and a growth rate equivalent t o that observed in serum-supplemented medium. Dibutyryl CAMP substitutes for prostaglandin E l in the medium, suggesting that increased growth of Maden Darby canine kidney cells results from increased intracellular CAMP. Potential applications of the serum-free medium are discussed. The medium permits the selective growth of primary epithelial cell cultures in the absence of fibroblast overgrowth, and a defined analysis of the mechanisms by which hormones regulate hemicyst formation.

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