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The serum-free growth of Balb/c 3T3 cells in medium supplemented with bovine colostrum

✍ Scribed by Klagsbrun, M. ;Neumann, J.

Publisher: Wiley (John Wiley & Sons)
Year: 1979
Tongue: English
Weight: 671 KB
Volume: 11
Category: Article
ISSN: 0091-7419
DOI: 10.1002/jss.400110310

No coin nor oath required. For personal study only.

✦ Synopsis

Bovine milk contains growth promoting factors that stimulate DNA synthesis and cell division in confluent monolayers of quiescent Balb/c 3T3 cells. The growth factor activity was highest in colostrum obtained within 24 hours after birth of a calf. Samples of milk obtained 3 2 hours and 60 hours after birth were 20'% and 1% as active respectively as was a sample obtained 8 hours after birth in stimulating DNA synthesis. No activity was detectable 3 days after hirth or thereafter. A similar temporal dependence was found in sheep's milk. Bovine colostrum obtained on the day of a calf's birth can be substituted for serum and will support the growth of sparse Balb/c 3 T 3 cells t o confluence. In Dulbecco's modified Eagle's medium (DMEM) supplemented with 2.5% (vol/vol) bovine colostrum, the number of Balb/c 3 T 3 cells in a dish increased 35-fold. from 2.0 X l o 4 cells to 7 X 10' cells. The generation time was approximately 38 hours. Proliferation of cells was characterized by formation of clusters of confluent Balb/c 3 T 3 cells which were smaller in size and more tightly packed than were Balb/c 3 T 3 cells grown t o confluence in serum. No proliferation was detected in DMEM supplemented with milk obtained 10 days after birth of a calf o r in DMEM supplemented with bovine serum albumen.

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