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Growth of mouse plasmacytoma cells in serum-free, hormone-supplemented medium: Procedure for the determination of hormone and growth factor requirements for cell growth

✍ Scribed by Hiroki Murakami; Hideo Masui; Gordon Sato; William C. Raschke

Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
676 KB
Volume
114
Category
Article
ISSN
0003-2697

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✦ Synopsis

The procedure of analyzing hormone and growth factor requirements for the growth of MPC-I I cells and of developing a serum-free medium for this cell line has been described. In this medium, MPC-I I cells grow as fast as in serum-supplemented medium, up to 50 generations.

MPC-I I cells grown in serum-supplemented medium secrete IgG,, and K light chain into the medium as they do in serum-containing medium.

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