The development of hormonally defined serum-free media (HDM) for the culture of mammalian cells has allowed the study of specific cellular functions in a totally defined environment. Recently, several reports have indicated that there are differences in t h e basic cellular physiology of cells cultu
Phospholipids regulate growth and function of MDCK cells in hormonally defined serum free medium
โ Scribed by Nabil Bashir; Kelli Kuhen; Mary Taub
- Book ID
- 112801650
- Publisher
- Society for In Vitro Biology
- Year
- 1992
- Tongue
- English
- Weight
- 617 KB
- Volume
- 28
- Category
- Article
- ISSN
- 1475-2689
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๐ SIMILAR VOLUMES
A chemically defined serum-free medium is described that has been optimized for the selective growth of immature astrocytes starting from early postnatal mouse cerebellar cultures. The medium is a modification of one described recently [Fischer et al, 1982] and consists of epidermal growth factor (E
Madin Darby canine kidney cells can grow in synthetic medium supplemented with 5 factors ~ insulin. transferrin, prostaglandin E , , hydrocortisone and triiodothyronine ~-as a seruni substitute. These 5 factors permit growth for one month in the absence of serum, and a growth rate equivalent t o tha