The pH range for optimal growth of WI-38 in Eagle's minimum essential medium varies with the carbohydrate utilized. Growth with glucose occurred only in the pH range 6.8-7.4. Glucose-free galactose supported growth only if the pH of the medium was adjusted to 6.4-6.8. Supplementing the galactose med
Propagation of the human diploid cell WI-38 in galactose medium
โ Scribed by Clarence L. Baugh; Alfred A. Tytell
- Book ID
- 118933871
- Publisher
- Elsevier Science
- Year
- 1967
- Tongue
- English
- Weight
- 351 KB
- Volume
- 6
- Category
- Article
- ISSN
- 0024-3205
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
Assessment of the respiratory and glycolytic capacity of non-growing WI-38 cells shows that, in the absence and presence of added glucose, the mean rates of oxygen consumption were 247 (QOz = 5.61) and 208 (QOz = 4.73) mpmoles/mg dry wt/hr., respectively. Mean glucose consumption was 225 mpmoles/mg
## Abstract By mapping the location of isolated single cells; and then counting the number of cells at each location as a function of time. it was possible to accumulate data on the growth history for each of a large group of clones. The clone size distribution, its mean and standard deviation were