Regulation of metastatic ovarian cell in serum-free medium by epidermal growth factor and transferrin

✍ Scribed by John T. Turner; James H. Wyche

• Publisher: John Wiley and Sons
• Year: 1980
• Tongue: English
• Weight: 550 KB
• Volume: 104
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041040213

✦ Synopsis

A rat ovarian tumorous cell line (OVlN) has been isolated and established in tissue culture whose growth can be controlled by the addition of epidermal growth factor (EGF) and transferrin to serum-free medium. EGF at 1 ng/ml (1.5 x 10-l0M) stimulates growth about 300% in a six-day incubation assay. A combination of 1 ng/ml EGF plus 1 pg/ml (1.25 x 10-8M) transferrin stimulates growth 1700% above controls in six days. EGF and transferrin a t these concentrations completely replaced fetal calf serum (FCS), which maximally stimulates the cells at 2%. At maximal stimulation (either by FCS or EGF and transferrin) a doubling time of from 16-20 hours is obtained. The growth of OVlN cells was also studied in vivo and compared to another rat ovarian cell line, 31A-F,. Cells from both cell lines were injected intrasplenically into normal, ovariectomized and hypophysectomized female Fischer 344 rats. Animal data have indicated that 31A-F, cells behave more like "normal" ovarian cells in that they grew only in ovariectomized hosts. OVlN cells, on the other hand, grew metastatically in all hosts and killed 36% of those individuals.

The binding of lZ5I-EGF was also studied in OVlN and 31A-F, cells. OVlN cells bound about eightfold more Iz5I-EGF than did 31A-F, cells at 37°C. The dose of lZ5I-EGF required to specifically saturate 31A-F, sites was 10 ng/ml (1.5