## Abstract Two inhibitors of somatic cell transmethylation, homocysteine thiolactone (Hcy) and 3βdeazadenosine (3βDZA), inhibited the acrosome reactions of hamster sperm in vitro. Hcy (250 ΞΌM) or 3βDZA (200 ΞΌM) inhibited acrosome reactions by approximately 30% after 4 hours of incubation with wash
Glycosaminoglycans stimulate the acrosome reaction of previously capacitated hamster sperm
β Scribed by Meizel, Stanley ;Turner, Kenneth O.
- Publisher
- John Wiley and Sons
- Year
- 1986
- Tongue
- English
- Weight
- 315 KB
- Volume
- 237
- Category
- Article
- ISSN
- 0022-104X
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β¦ Synopsis
The glycosaminoglycans (GAGs) hyaluronic acid and heparin were added (10 pg and 100 pglml to golden hamster sperm suspensions previously incubated for 4.5 h under capacitating conditions. After additions, sperm were incubated for 5-15 min and acrosome reactions (AR) assayed in motile sperm by phase contrast microscopy. Hyaluronic acid and heparin significantly
π SIMILAR VOLUMES
This study describes investigations of the importance of intraacrosomal pH in the hamster sperm acrosome reaction (AR). Washed cauda epididymal sperm were capacitated in vitro in a medium containing 2 mM Ca2+, 144 mM Na+, and 3 mM K + . Such sperm underwent a significant increase in the number of AR
## Abstract Phospholipase A~2~ and lysophospholipids have been implicated in the mammalian sperm acrosome reaction. In this study we further investigated the role of this enzyme and lysophospholipids on the acrosome reaction of hamster spermatozoa. Hamster epididymal spermatozoa were incubated unde
## Abstract Acrosome reactions occurring in vitro in hamster sperm capacitated by bovine follicular fluid were severely inhibited by four synthetic trypsin inhibitors and by Zn^2^ +. Three polypeptide trypsin inhibitors and a synthetic chymotrypsin inhibitor did not inhibit the acrosome reaction, a
## Abstract ABSTRACT The mammalian sperm acrosome reaction (AR) is a fusion and fenestration of sperm head membranes which is essential for fertilization. Our earlier work demonstrated that arachidonic acid could stimulate the AR 15 min after addition to hamster sperm capacitated by incubation for