Correlation of increased intraacrosomal pH with the hamster sperm acrosome reaction

✍ Scribed by Working, Peter K. ;Meizel, Stanley

• Publisher: John Wiley and Sons
• Year: 1983
• Tongue: English
• Weight: 957 KB
• Volume: 227
• Category: Article
• ISSN: 0022-104X
• DOI: 10.1002/jez.1402270114

✦ Synopsis

This study describes investigations of the importance of intraacrosomal pH in the hamster sperm acrosome reaction (AR). Washed cauda epididymal sperm were capacitated in vitro in a medium containing 2 mM Ca2+, 144 mM Na+, and 3 mM K + . Such sperm underwent a significant increase in the number of AR within 10 min after the addition of the Mg2+-ATPase (adenosine triphosphatase) inhibitors DCCD (20 pM) or NBD-C1 (10 pM) or the proton ionophore FCCP (6 pgiml) at 3.5 hr of incubation or after addition of NHdCl(3 mM) at 4 hr of incubation. Addition of the mitochondria1 electron transport inhibitor rotenone (2.5 pM) at 3.5 hr or of NaCl (3 mM) or KCl (3 mM) at 4 hr did not stimulate AR over control levels, suggesting that the stimulation of AR by the other compounds was not directly due to depletion of acrosomal adenosine triphosphate (ATP) or alteration of the acrosomal transmembrane potential. The AR also was not stimulated by either DCCD or FCCP added prior to 3 hr of incubation of sperm, whereas both compounds were increasingly effective at stimulating AR with increasing length of preincubation of sperm before the addition of the test compounds.

The intraacrosomal pH of sperm incubated in low [K+] (0.6-0.9 mM) for 3.5 hr rose by a t least one pH unit (as measured with the fluorescent dye 9aminoacridine) within 15-30 min after raising extracellular [K'] to 4.2-4.5 mM. The pH rise occurred even in the presence of the Ca2+-chelator EGTA (2 mM). Either FCCP (8pg/ml) or DCCD (20 pM), but not rotenone (2.5 pM), plus K + (3.6 mM), raised the intraacrosomal pH of sperm incubated for 3 hr in low