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Inhibition of the hamster sperm acrosome reaction by transmethylation inhibitors

✍ Scribed by Meizel, Stanley

Publisher
John Wiley and Sons
Year
1981
Tongue
English
Weight
331 KB
Volume
217
Category
Article
ISSN
0022-104X

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✦ Synopsis

Abstract

Two inhibitors of somatic cell transmethylation, homocysteine thiolactone (Hcy) and 3–deazadenosine (3–DZA), inhibited the acrosome reactions of hamster sperm in vitro. Hcy (250 ΞΌM) or 3–DZA (200 ΞΌM) inhibited acrosome reactions by approximately 30% after 4 hours of incubation with washed sperm in a capacitating medium containing epinephrine, taurine, and bovine serum albumin. Inhibition by 3–DZA decreased by 5 hours, but inhibition by Hcy did not. Incubation of sperm with the combination of both inhibitors inhibited acrosome reactions by nearly 60% at 4 hours, and did not appreciably decrease with time up to 5 hours. These results are the first to demonstrate that such inhibitors can interfere with the acrosome reaction and taken together with the effects of Hcy and 3 – DZA on somatic cells, suggest that sperm protein and/or phospholipid transmethylation is/are involved in capacitation and/or the acrosome reaction.

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