Genetic analysis workshop III: Multipoint linkage analysis using FAP
โ Scribed by Max P. Baur; Meinhard Neugebauer; Martin Sigmund
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 141 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0741-0395
No coin nor oath required. For personal study only.
โฆ Synopsis
The family analysis package (FAP), originally designed for the analysis of the multilocus pedigree data of the 9th HLA workshop, was applied to the problem of multipoint mapping and estimation from the simulated data of GAW III. The fourpoint analyses performed within the two identified linkage groups yielded correct results.
๐ SIMILAR VOLUMES
Two linkage groups were identified through pairwise linkage analysis using PAP. Three-locus analysis confirmed the suggested order of the loci within the linkage groups and was used to assess the strength of the conclusions. Strategies for improving the efficiency of linkage analysis are discussed.
A three-ste;, procedure was employed to analyze the workshop data. Pairwise lod scores were computed using LIPED and were utilized for estimating the recombination frequencies and thereby the order among loci. This approach proved sugcient to identify accurately and to order the two linkage groups.
Familial segregation and linkage analyses were performed on two sets of the Genetic Analyses Workshop II data. The salient features of the mode of inheritance of the disease trait and its linkage/association with polymorphic markers and also marker-marker linkages were delineated using statistical-g
Linkage groups and orders are established through two-point analysis; gene order is verified through simple three-point analysis; linkage maps are obtained through weighted least squares, fitting observed 8 values to those predicted by a parametric mapping function.
Two-point linkage analyses yielded correct identification of linkage groups and good estimates of recombination frequencies in both males and females. Additional analyses compared various family structures for the total number of typed individuals necessary to detect linkage. Results suggest that fo