Genetic analysis workshop III: Two-point analysis of multipoint data
โ Scribed by M. Anne Spence; Mary L. Marazita; Susan E. Hodge
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 131 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0741-0395
No coin nor oath required. For personal study only.
โฆ Synopsis
A three-ste;, procedure was employed to analyze the workshop data. Pairwise lod scores were computed using LIPED and were utilized for estimating the recombination frequencies and thereby the order among loci. This approach proved sugcient to identify accurately and to order the two linkage groups. Coincidence and interference were estimated. Associations between the alleles were tested. The following is a brief summary of this study. Detailed results or program listings are available upon request.
๐ SIMILAR VOLUMES
The family analysis package (FAP), originally designed for the analysis of the multilocus pedigree data of the 9th HLA workshop, was applied to the problem of multipoint mapping and estimation from the simulated data of GAW III. The fourpoint analyses performed within the two identified linkage grou
Linkage groups and orders are established through two-point analysis; gene order is verified through simple three-point analysis; linkage maps are obtained through weighted least squares, fitting observed 8 values to those predicted by a parametric mapping function.
A method to construct genetic maps using two-point lod tables was applied to the simulated pedigree data. The analysis provided the correct map order and distances between markers with remarkable accuracy. The methodology is discussed.
Two-point linkage analyses yielded correct identification of linkage groups and good estimates of recombination frequencies in both males and females. Additional analyses compared various family structures for the total number of typed individuals necessary to detect linkage. Results suggest that fo
The participants of Presentation Group 1 used the GAW13 data to derive new phenotypes, which were then analyzed for linkage and, in one case, for association to the genetic markers. Since the trait measurements ranged over longer time periods, the participants looked at the time dependence of partic