A three-ste;, procedure was employed to analyze the workshop data. Pairwise lod scores were computed using LIPED and were utilized for estimating the recombination frequencies and thereby the order among loci. This approach proved sugcient to identify accurately and to order the two linkage groups.
Genetic analysis workshop III: Construction of genetic maps using two-point lod tables
โ Scribed by S. L. Sherman; N. E. Morton
- Publisher
- John Wiley and Sons
- Year
- 1985
- Tongue
- English
- Weight
- 118 KB
- Volume
- 2
- Category
- Article
- ISSN
- 0741-0395
No coin nor oath required. For personal study only.
โฆ Synopsis
A method to construct genetic maps using two-point lod tables was applied to the simulated pedigree data. The analysis provided the correct map order and distances between markers with remarkable accuracy. The methodology is discussed.
๐ SIMILAR VOLUMES
Two-point methods were used throughout. Male and female lod scores were calculated using LIPED. Edwards' programs, ZLODS and OBM, were used for joint analysis of the lod scores. ZLODS estimates recombination fractions. OBM calculates the likelihood and estimates the map distances for a given orderin
Two-point linkage analyses yielded correct identification of linkage groups and good estimates of recombination frequencies in both males and females. Additional analyses compared various family structures for the total number of typed individuals necessary to detect linkage. Results suggest that fo
For each pair of loci among the four loci in a linkage group, a two-point analysis was performed. It consisted of estimating means, variances, and correlation of male and female recombination fractions and approximating the joint likelihood by a bivariate normal density. The painvise results were as