Generation of superoxide anion by brain endothelial cell xanthine oxidase

✍ Scribed by Lance S. Terada; Irene R. Willingham; Mary E. Rosandich; Jonathan A. Leff; Glenn W. Kindt; John E. Repine

• Book ID: 102887021
• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 655 KB
• Volume: 148
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041480202

✦ Synopsis

Bovine brain endothelial cells (EC) that were isolated and propagated in pure culture had increased (> 20-fold) levels of xanthine oxidase and xanthine dehydrogenase activity compared to whole brain homogenate. Brain EC also released superoxide anion (0;) into the extracellular medium. Treatment of EC with tungsten decreased (P < 0.05) both XO activity and 0, release. XO appears to be highly concentrated in cerebral vascular endothelium and may be an important source of 0 ,