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Fractionation of nuclei from Paramecium aurelia by density gradient centrifugation

✍ Scribed by Russell E. Isaacks; Berta G. Santos; Willem J. Van Wagtendonk

Book ID
115743702
Publisher
Elsevier Science
Year
1969
Weight
432 KB
Volume
195
Category
Article
ISSN
0005-2787

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A simple effective technique for the fractionation of protoplast populations is described. Protoplasts are separated by low-speed centrifugation in an iso-osmotic, discontinuous density gradient system on the basis of differences in their buoyant densities. At a constant osmolality of 660Β±20 mOs/kg