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Analytical subcellular fractionation of normal human skeletal muscle by sucrose density gradient centrifugation

โœ Scribed by F. C. MARTIN; A. J. LEVI; G. SLAVIN; T. J. PETERS


Book ID
114726282
Publisher
John Wiley and Sons
Year
1983
Tongue
English
Weight
695 KB
Volume
13
Category
Article
ISSN
0014-2972

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## Mitochondria prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins. These contaminants can be removed by density-gradient centrifugation in Percoll. The resulting mitochondria retain both their original state three respiratory