๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

Purification of skeletal-muscle mitochondria by density-gradient centrifugation with Percoll

โœ Scribed by James R. Mickelson; Marion L. Greaser; B.Bruce Marsh

Publisher
Elsevier Science
Year
1980
Tongue
English
Weight
864 KB
Volume
109
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

Mitochondria

prepared from skeletal muscle are typically contaminated with sarcoplasmic reticulum fragments and salt-soluble proteins.

These contaminants can be removed by density-gradient centrifugation in Percoll.

The resulting mitochondria retain both their original state three respiratory rates and their respiratory control ratios. The method has also been adapted to prepare mitochondria from very small tissue samples.

๐Ÿ“œ SIMILAR VOLUMES

Purification of HBsAg from human placent
Purification of HBsAg from human placental blood by density-gradient zonal centrifugation
โœ Yan-Bo Xie; Ji-Zhang Wang; Guo-Quiang Zhang; Li-Xin Wang ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› John Wiley and Sons ๐ŸŒ English โš– 926 KB

Highly purified preparations of HBsAg are urgently needed for the production of hepatitis B diagnostic products and vaccines. The raw material for the production of HBsAg usually involves blood from HBsAg-positive carriers. In some countries, it is difficult to draw blood from such carriers due to l

Interaction of antibody with antigen imm
Interaction of antibody with antigen immobilized on polystyrene latex beads: Characterization by density gradient centrifugation
โœ Richard K. Genung; Hsien-Wen Hsu ๐Ÿ“‚ Article ๐Ÿ“… 1978 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 692 KB

Isopycnic banding by density gradient centrifugation was used to measure density changes in complexes formed by the interaction between antigen and antibody immobilized on polystyrene latex beads (diameter, 0.109 +/- 0.0025 micron). Measurements of density changes allowed calculation of the interact