Interaction of antibody with antigen immobilized on polystyrene latex beads: Characterization by density gradient centrifugation

✍ Scribed by Richard K. Genung; Hsien-Wen Hsu

• Publisher: Elsevier Science
• Year: 1978
• Tongue: English
• Weight: 692 KB
• Volume: 91
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(78)90552-3

✦ Synopsis

Isopycnic banding by density gradient centrifugation was used to measure density changes in complexes formed by the interaction between antigen and antibody immobilized on polystyrene latex beads (diameter, 0.109 +/- 0.0025 micron). Measurements of density changes allowed calculation of the interacting masses under the given experimental conditions. Interaction equilibrium constants and free energy change for two sets of reactions, bovine IgG and anti-bovine IgG (rabbit) IgG and rabbit IgG and anti-rabbit IgG (goat) IgG systems, were calculated from isopycnic banding density gradient centrifugation runs. The procedure demonstrates a new method of obtaining quantitative information on antigen-antibody interactions.