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Fractionation of plant protoplast types by iso-osmotic density gradient centrifugation

✍ Scribed by Ch. T. Harms; I. Potrykus

Book ID
104954272
Publisher
Springer
Year
1978
Tongue
English
Weight
724 KB
Volume
53
Category
Article
ISSN
0040-5752

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πŸ“œ SIMILAR VOLUMES

Fractionation of plant protoplast types
Fractionation of plant protoplast types by iso-osmotic density gradient centrifugation
✍ Ch. T. Harms; I. Potrykus πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 724 KB

A simple effective technique for the fractionation of protoplast populations is described. Protoplasts are separated by low-speed centrifugation in an iso-osmotic, discontinuous density gradient system on the basis of differences in their buoyant densities. At a constant osmolality of 660Β±20 mOs/kg

Enrichment for heterokaryocytes by the u
Enrichment for heterokaryocytes by the use of iso-osmotic density gradients after plant protoplast fusion
✍ Ch. T. Harms; I. Potrykus πŸ“‚ Article πŸ“… 1978 πŸ› Springer 🌐 English βš– 1003 KB

Buoyant density differences between different types of protoplasts were used in an iso-osmotic density gradient system to enrich protoplast fusion mixtures for heterokaryocytes. Protoplasts of maize stem and wheat mesophyll, as well as epidermis, stem parenchyma and mesophyll protoplasts of two amph