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Fractionation of calf thymus deoxyribonucleic acid by density-gradient centrifugation

✍ Scribed by E. Polli; G. Corneo; E. Ginelli; P. Bianchi


Book ID
115741858
Publisher
Elsevier Science
Year
1965
Weight
300 KB
Volume
103
Category
Article
ISSN
0005-2787

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A simple effective technique for the fractionation of protoplast populations is described. Protoplasts are separated by low-speed centrifugation in an iso-osmotic, discontinuous density gradient system on the basis of differences in their buoyant densities. At a constant osmolality of 660Β±20 mOs/kg