Fluorometric determination of trioses with o-phenylphenol

✍ Scribed by Yoshihiro Asabe; Shigeyoshi Momose; Masao Suzuki; Shoji Takitani

• Publisher: Elsevier Science
• Year: 1979
• Tongue: English
• Weight: 399 KB
• Volume: 94
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(79)90781-4

✦ Synopsis

It was found that trioses, such as glyceraldehyde and dihydroxyacetone, could be determined by using the assay for acetaldehyde reported earlier, on the basis of the fluorescence reaction between acetaldehyde and o-phenylphenol in sulfuric acid. The calibration curves for the standard solutions of glyceraldehyde and dihydroxyacetone showed straight lines passing through the origin with the same slope for 1.8-72 nmoU200 ~1. Then, the standard method for the assay of trioses, acetaldehyde, and lactic acid in mixed sample solutions was established by a combination of separation of lactic acid from the other two components with an anion-exchange resin treatment and simultaneous determination of trioses and acetaldehyde based upon the differences in the reaction condition between the both compounds. Finally, almost 100% recovery was obtained for each of the components in the examination of the recovery of these three compounds added to the chemistry control serum. ' This paper constitutes Part V of a series entitled "Color Reactions of Carbonyl Compounds with Substituted-phenols"; Part IV in the series is Ref. (1).