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Fluorometric determination of carbohydrate with 2-aminothiophenol

✍ Scribed by Jian-Kang Zhu; Eugene A. Nothnagel

Publisher: Elsevier Science
Year: 1991
Tongue: English
Weight: 404 KB
Volume: 195
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(91)90302-a

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✦ Synopsis

The 2-aminothiophenol-based fluorometric assay of Nakano et al. (1973, J. Pharm. Soc. Jpn. 93, 350-353) for monosaccharides has been modified to improve the speed, applicability, and sensitivity of the method. The improved assay is applicable to complex carbohydrates as well as to monosaccharides. Less than 50 ng of carbohydrate in a final volume of 2 ml can be quantitatively measured within 30 min. The assay is reasonably compatible with the presence of a variety of reagents commonly used in aqueous buffer solutions. The assay is especially useful for monitoring column eluents during the purification of small quantities of carbohydrates or their conjugates.

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