Fluorescence of the Schiff bases of pyridoxal and pyridoxal 5′-phosphate withl-isoleucine in aqueous solutions
✍ Scribed by Gregoria Cambrón; José M. Sevilla; Teresa Pineda; Manuel Blázquez
- Publisher
- Springer
- Year
- 1996
- Tongue
- English
- Weight
- 501 KB
- Volume
- 6
- Category
- Article
- ISSN
- 1053-0509
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📜 SIMILAR VOLUMES
Simultaneous and direct assays of pyridoxal, pyridoxal-5'-phosphate, and pyridoxic acid in human serum are described. The method applied is based on the reaction of these compounds with beryllium in an ammoniacal medium to yield highly fluorescent derivatives. Overlapping of conventional fluorescenc
The formation constants of the Schiff bases of pyridoxal %-phosphate with polyallylamine were determined over the pH range of the acetic-acetate buffer (3.9-5.5) a t an ionic strength of 0.1 M and a temperature of 25°C. The results were consistent with the rapid formation of an ionized carbinolamine
We determined the apparent equilibrium constant of formation, KpH, of the Schijf bases of pyridoxal 5'-phosphate (PLP) and poly-and copolymers containing L-lysine, as a function of pH at 25" and a constant ionic strength of 0 . 1 ~. The KPH values obtained at acidic and neutral pH were larger that t