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Schiff bases of pyridoxal 5′-phosphate and polypeptides containing L-lysine: A kinetic study

✍ Scribed by M.Angeles Garcia del Vado; Gerardo R. Echevarria; Francisco García Blanco; José G. Santos Blanco; Manuel Blázquez; José Manuel Sevilla; Manuel Dominguez

Publisher
Elsevier Science
Year
1991
Weight
516 KB
Volume
68
Category
Article
ISSN
0304-5102

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We determined the apparent equilibrium constant of formation, KpH, of the Schijf bases of pyridoxal 5'-phosphate (PLP) and poly-and copolymers containing L-lysine, as a function of pH at 25" and a constant ionic strength of 0 . 1 ~. The KPH values obtained at acidic and neutral pH were larger that t

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We studied the formation and hydrolysis of the Schiff base of pyridoxal-5'-phosphate (PLP) and n-dodecylamine (DOD) by kinetic methods and found the formation rate constants to be much larger than those reported for related systems. This can be ascribed to the occurrence of local concentration of ch