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On the Stability of the Schiff Bases of Pyridoxal 5′-Phosphate with Polypeptides Containing L-Lysine

✍ Scribed by M. Angeles García del Vado; Gerardo R. Echevarria; Francisco García Blanco; José G. Santos Blanco; José Laynez Vallejo; José L. García de Paz

Publisher
John Wiley and Sons
Year
1991
Tongue
German
Weight
506 KB
Volume
74
Category
Article
ISSN
0018-019X

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✦ Synopsis

We determined the apparent equilibrium constant of formation, KpH, of the Schijf bases of pyridoxal 5'-phosphate (PLP) and poly-and copolymers containing L-lysine, as a function of pH at 25" and a constant ionic strength of 0 . 1 ~. The KPH values obtained at acidic and neutral pH were larger that those reported for Sclzij'bases of PLP and hexylamine. We determined calorimetrically A H of formation of Schiff bases of PLP and poly(L-lysine) (-4.5 kcal/mol), and PLP and hexylamine (-3.4 kcal/mol) at pH 7.00. Semi-empirical theoretical calculations (INDO and AM1 methods) of a model compound of Schijj base of PLP and polypeptide containing L-lysine show the capability of specific interactions between groups of PLP and the peptide skeleton.

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