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Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes

โœ Scribed by Sally S. Twining

Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
373 KB
Volume
143
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A simple inexpensive sensitive protease assay was developed using soluble fluorescein isothiocyanate (FITC)-labeled casein. Casein was reacted with FITC to form the fluorescein thiocarbamoyl derivative. This substrate is cleaved by trypsin, chymotrypsin, elastase, subtilisin, and thermolysin in a linear time-dependent manner. These enzymes can be measured in the nanogram and subnanogram range using this assay. The assay is reproducible, has a low blank, and uses casein, which resembles natural substrates of most proteases.

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