๐”– Bobbio Scriptorium
โœฆ   LIBER   โœฆ

A fluorescent assay for proteolytic enzymes

โœ Scribed by Christian Schwabe

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
418 KB
Volume
53
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

โœฆ Synopsis

A method is described which permits the assay of proteolytic enzyme activity on protein substrates without precipitation or filtration steps, utilizing a fluorescent reagent which is specific for primary amines. The assay is about 100 times more sensitive than the Lowry method, much faster and less complicated. Ambiguities concerning the absorbing species are largely eliminated. The reagent (Fluorescamine.

Hoffmann-La Roche RO-20-7234

๐Ÿ“œ SIMILAR VOLUMES

Fluorescein isothiocyanate-labeled casei
Fluorescein isothiocyanate-labeled casein assay for proteolytic enzymes
โœ Sally S. Twining ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 373 KB

A simple inexpensive sensitive protease assay was developed using soluble fluorescein isothiocyanate (FITC)-labeled casein. Casein was reacted with FITC to form the fluorescein thiocarbamoyl derivative. This substrate is cleaved by trypsin, chymotrypsin, elastase, subtilisin, and thermolysin in a li

A rapid method for the quantitative assa
A rapid method for the quantitative assay of proteolytic enzymes
โœ Walter L. Nelson; Edward I. Ciaccio; George P. Hess ๐Ÿ“‚ Article ๐Ÿ“… 1961 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 325 KB

Insoluble dye-protein complexes offer many advantages when used as substrates for proteolytie enzymes. The proteolytic split products can be separated from the starting substrat.e conveniently by filtration, and the concentration of the soluble colored products of hydrolysis can easily be measured a

An Assay for Detecting Nanogram Levels o
An Assay for Detecting Nanogram Levels of Proteolytic Enzymes
โœ V.M. Koritsas; H.J. Atkinson ๐Ÿ“‚ Article ๐Ÿ“… 1995 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 404 KB

A rapid, cheap, and sensitive method has been developed for determining proteolytic activity of different classes of endoproteinases. The method is based on a solid-phase assay employing as substrate biotinylated gelatin adsorbed onto microtiter plates. Enzymatic activity is measured by incubating p