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A sensitive assay for proteolytic enzymes using bacterial luciferase as a substrate

โœ Scribed by David Njus; Thomas O. Baldwin; J.W. Hastings

Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
488 KB
Volume
61
Category
Article
ISSN
0003-2697

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A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By m