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A sensitive microplate assay for the detection of proteolytic enzymes using radiolabeled gelatin

โœ Scribed by B.D. Robertson; G.E. Kwan-Lim; R.M. Maizels

Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
365 KB
Volume
172
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A sensitive, microplate assay is described for the detection of a wide range of proteolytic enzymes, using radio-iodine-labeled gelatin as substrate. The technique uses the Bolton-Hunter reagent to label the substrate, which is then coated onto the wells of polyvinyl chloride microtiter plates. By measuring the radioactivity released the assay is able to detect elastase, trypsin, and collagenase in concentrations of 1 ng/ml or less, while the microtiter format permits multiple sample handling and minimizes sample volumes required for analysis.

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